Paternal Genome Elimination in Liposcelis Booklice (Insecta: Psocodea)

dc.contributor.authorHodson, Christina
dc.contributor.authorHamilton, Phineas
dc.contributor.authorDilworth, Dave
dc.contributor.authorNelson, Chris
dc.contributor.authorCurtis, Caitlin I.
dc.contributor.authorPerlman, Steve
dc.date.accessioned2017-07-31T17:27:14Z
dc.date.available2017-07-31T17:27:14Z
dc.date.copyright2017en_US
dc.date.issued2017-06-01
dc.description.abstractHow sex is determined in insects is diverse and dynamic, and includes male heterogamety, female heterogamety, and haplodiploidy. In many insect lineages, sex determination is either completely unknown or poorly studied. We studied sex determination in Psocodea—a species-rich order of insects that includes parasitic lice, barklice, and booklice. We focus on a recently discovered species of Liposcelis booklice (Psocodea: Troctomorpha), which are among the closest free-living relatives of parasitic lice. Using genetic, genomic, and immunohistochemical approaches, we show that this group exhibits paternal genome elimination (PGE), an unusual mode of sex determination that involves genomic imprinting. Controlled crosses, following a genetic marker over multiple generations, demonstrated that males only transmit to offspring genes they inherited from their mother. Immunofluorescence microscopy revealed densely packed chromocenters associated with H3K9me3—a conserved marker for heterochromatin—in males, but not in females, suggesting silencing of chromosomes in males. Genome assembly and comparison of read coverage in male and female libraries showed no evidence for differentiated sex chromosomes. We also found that females produce more sons early in life, consistent with facultative sex allocation. It is likely that PGE is widespread in Psocodea, including human lice. This order represents a promising model for studying this enigmatic mode of sex determination.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis work was supported by a National Sciences and Engineering Council of Canada Discovery Grant to SP. SP acknowledges support from the Integrated Microbial Biodiversity Program of the Canadian Institute for Advanced Research.en_US
dc.identifier.citationHodson, C.N., Hamilton, P.T., Dilworth, D., Nelson, C.J., Curtis, C.I. & Perlman, S.J. (2017). Paternal Genome Elimination in Liposcelis Booklice (Insecta: Psocodea). Genetics, 206(2), 1091-1100. doi.org/10.1534/genetics.117.199786en_US
dc.identifier.urihttps://doi.org/10.1534/genetics.117.199786
dc.identifier.urihttp://hdl.handle.net/1828/8386
dc.language.isoenen_US
dc.publisherGeneticsen_US
dc.subjectgenome exclusionen_US
dc.subjectsegregation distortionen_US
dc.subjectsex determinationen_US
dc.subjectsex ratioen_US
dc.subjectgenomic imprintingen_US
dc.titlePaternal Genome Elimination in Liposcelis Booklice (Insecta: Psocodea)en_US
dc.typePreprinten_US

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Hodson_CN_Genetics_2017.pdf
Size:
4.3 MB
Format:
Adobe Portable Document Format
Description:
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description: