N-3 PUFA deficiency affects the ultrastructural organization and density of white matter microglia in the developing brain of male mice

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dc.contributor.authorDecoeur, Fanny
dc.contributor.authorPicard, Katherine
dc.contributor.authorSt-Pierre, Marie-Kim
dc.contributor.authorGreenhalgh, Andrew D.
dc.contributor.authorDelpech, Jean-Christophe
dc.contributor.authorSere, Alexandra
dc.contributor.authorLayé, Sophie
dc.contributor.authorTremblay, Marie-Ève
dc.contributor.authorNadjar, Agnès
dc.date.accessioned2024-02-09T16:09:49Z
dc.date.available2024-02-09T16:09:49Z
dc.date.copyright2022en_US
dc.date.issued2022
dc.descriptionWe would like to thank Patrick Nahirney and Brent Gowen for the access to the transmission electron microscope. We would also like to thank Christel Poujol, Sébastien Marais, Fabrice Cordelières, Jérémie Teillon,Magali Mondin, Monica Fernandez-Monreal from the Bordeaux Imaging Center (Bordeaux, France) for their help with microscopy experiments and image analysis. We like to thank Maude Bordeleau for her help with image analyses. We finally thank the animal facility of the NutriNeuro lab for taking care of and providing the animals that were necessary to perform the experiments. We acknowledge and respect the Lək̓ʷəŋən people on whose traditional territory the University of Victoria stands and the Songhees, Esquimalt and W̱SÁNEĆ peoples whose historical relationships with the land continue to this day.en_US
dc.description.abstractOver the last century, westernization of dietary habits has led to a dramatic reduction in dietary intake of n-3 polyunsaturated fatty acids (n-3 PUFAs). In particular, low maternal intake of n-3 PUFAs throughout gestation and lactation causes defects in brain myelination. Microglia are recognized for their critical contribution to neurodevelopmental processes, such as myelination. These cells invade the white matter in the first weeks of the post-natal period, where they participate in oligodendrocyte maturation and myelin production. Therefore, we investigated whether an alteration of white matter microglia accompanies the myelination deficits observed in the brain of n-3 PUFA-deficient animals. Macroscopic imaging analysis shows that maternal n-3 PUFA deficiency decreases the density of white matter microglia around post-natal day 10. Microscopic electron microscopy analyses also revealed alterations of microglial ultrastructure, a decrease in the number of contacts between microglia and myelin sheet, and a decreased amount of myelin debris in their cell body. White matter microglia further displayed increased mitochondrial abundance and network area under perinatal n-3 PUFA deficiency. Overall, our data suggest that maternal n-3 PUFA deficiency alters the structure and function of microglial cells located in the white matter of pups early in life, and this could be the key to understand myelination deficits during neurodevelopment.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis research was funded by the Institut National pour la Recherche Agronomique, l’Alimentation et l’Environnement (INRAE), the Bordeaux Univ and the International Laboratory OptiNutriBrain. AN was supported by the Region Ile de France (PICRI, the Cerebral Palsy Foundation #13020605) and by the FRM. M-ET holds a Canada Research Chair (Tier 2) in Neurobiology of Aging and Cognition. AN and M-ET were supported by the Fonds de Recherche du Québec- Nature et Technologie (FRQNT). SL was supported by the Foundation for Medical Research (FRM, DEQ20170336724), the French Foundation (FDF, #00070700), Fondation pour la Recherche sur le Cerveau (FRC), Fondation Carrefour and Région Nouvelle Aquitaine. FD was funded by Idex/University of Bordeaux and benefited from the Bordeaux Neurocampus Graduate Program (EUR 17-EURE-0028 project). KP was supported by a doctoral scholarship from Fonds de recherche du Québec—Santé (FRQS). M-KS-P was supported by a doctoral training award from the Canadian Institutes of Health Research (CIHR) and a Fond de Recherche du Québec-Santé (FRQS) doctoral award. Bordeaux Imaging Center a service unit of the CNRS-INSERM and Bordeaux University, member of the national infrastructure France BioImaging, are supported by the French National Research Agency (ANR-10-INBS-04).en_US
dc.identifier.citationDecoeur, F., Picard, K., St-Pierre, M-K., Greenhalgh, A. D., Delpech, J-C., Sere, A., ... Nadjar, A. (2022). N-3 PUFA deficiency affects the ultrastructural organization and density of white matter microglia in the developing brain of male mice. Frontiers in Cellular Neuroscience, 16, 802411. https://doi.org/10.3389/fncel.2022.802411en_US
dc.identifier.urihttps://doi.org/10.3389/fncel.2022.802411
dc.identifier.urihttp://hdl.handle.net/1828/15977
dc.language.isoenen_US
dc.publisherFrontiers in Cellular Neuroscienceen_US
dc.subjectn-3 PUFAen_US
dc.subjectmicrogliaen_US
dc.subjectmyelinen_US
dc.subjectneurodevelopmenten_US
dc.subjectelectron microscopyen_US
dc.titleN-3 PUFA deficiency affects the ultrastructural organization and density of white matter microglia in the developing brain of male miceen_US
dc.typeArticleen_US

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We acknowledge and respect the Lək̓ʷəŋən (Songhees and Esquimalt) Peoples on whose territory the university stands, and the Lək̓ʷəŋən and W̱SÁNEĆ Peoples whose historical relationships with the land continue to this day.