Toward increased reliability, transparency, and accessibility in cross-linking mass spectrometry

Date

2020

Authors

Leitner, Alexander
Bonvin, Alexandre M. J. J.
Borchers, Christoph H.
Chalkley, Robert J.
Chamot-Rooke, Julia
Combe, Colin W.
Cox, Jürgen
Dong, Meng-Qiu
Fischer, Lutz
Götze, Michael

Journal Title

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Volume Title

Publisher

Structure

Abstract

Cross-linking mass spectrometry (MS) has substantially matured as a method over the past 2 decades through parallel development in multiple labs, demonstrating its applicability to protein structure determination, conformation analysis, and mapping protein interactions in complex mixtures. Cross-linking MS has become a much-appreciated and routinely applied tool, especially in structural biology. Therefore, it is timely that the community commits to the development of methodological and reporting standards. This white paper builds on an open process comprising a number of events at community conferences since 2015 and identifies aspects of Cross-linking MS for which guidelines should be developed as part of a Cross-linking MS standards initiative.

Description

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Citation

Leitner, A., Bonvin, A. M. J. J., Borchers, C. H., Chalkley, R. J., Chamot‐Rooke, J., Combe, C., Cox, J., Dong, M., Fischer, L., Gøtze, M., Gozzo, F. C., Heck, A. J. R., Hoopmann, M. R., Huang, L., Ishihama, Y., Jones, A. R., Kalisman, N., Kohlbacher, O., Mechtler, K., . . . Rappsilber, J. (2020). Toward increased reliability, transparency, and accessibility in cross-linking mass spectrometry. Structure, 28(11), 1259–1268. https://doi.org/10.1016/j.str.2020.09.011