Toward increased reliability, transparency, and accessibility in cross-linking mass spectrometry
Date
2020
Authors
Leitner, Alexander
Bonvin, Alexandre M. J. J.
Borchers, Christoph H.
Chalkley, Robert J.
Chamot-Rooke, Julia
Combe, Colin W.
Cox, Jürgen
Dong, Meng-Qiu
Fischer, Lutz
Götze, Michael
Journal Title
Journal ISSN
Volume Title
Publisher
Structure
Abstract
Cross-linking mass spectrometry (MS) has substantially matured as a method over the past 2 decades through parallel development in multiple labs, demonstrating its applicability to protein structure determination, conformation analysis, and mapping protein interactions in complex mixtures. Cross-linking MS has become a much-appreciated and routinely applied tool, especially in structural biology. Therefore, it is timely that the community commits to the development of methodological and reporting standards. This white paper builds on an open process comprising a number of events at community conferences since 2015 and identifies aspects of Cross-linking MS for which guidelines should be developed as part of a Cross-linking MS standards initiative.
Description
Keywords
Citation
Leitner, A., Bonvin, A. M. J. J., Borchers, C. H., Chalkley, R. J., Chamot‐Rooke, J., Combe, C., Cox, J., Dong, M., Fischer, L., Gøtze, M., Gozzo, F. C., Heck, A. J. R., Hoopmann, M. R., Huang, L., Ishihama, Y., Jones, A. R., Kalisman, N., Kohlbacher, O., Mechtler, K., . . . Rappsilber, J. (2020). Toward increased reliability, transparency, and accessibility in cross-linking mass spectrometry. Structure, 28(11), 1259–1268. https://doi.org/10.1016/j.str.2020.09.011