Microfluidic-based oxygen (O2) sensors for on-chip monitoring of cell, tissue and organ metabolism
Date
2021
Authors
Azimzadeh, Mostafa
Khashayar, Patricia
Amereh, Meitham
Tasnim, Nishat
Hoorfar, Mina
Akbari, Mohsen
Journal Title
Journal ISSN
Volume Title
Publisher
Biosensors
Abstract
Oxygen (O2) quantification is essential for assessing cell metabolism, and its consumption
in cell culture is an important indicator of cell viability. Recent advances in microfluidics have made
O2 sensing a crucial feature for organ-on-chip (OOC) devices for various biomedical applications.
OOC O2 sensors can be categorized, based on their transducer type, into two main groups, optical
and electrochemical. In this review, we provide an overview of on-chip O2 sensors integrated with
the OOC devices and evaluate their advantages and disadvantages. Recent innovations in optical O2
sensors integrated with OOCs are discussed in four main categories: (i) basic luminescence-based
sensors; (ii) microparticle-based sensors; (iii) nano-enabled sensors; and (iv) commercial probes and
portable devices. Furthermore, we discuss recent advancements in electrochemical sensors in five
main categories: (i) novel configurations in Clark-type sensors; (ii) novel materials (e.g., polymers, O2
scavenging and passivation materials); (iii) nano-enabled electrochemical sensors; (iv) novel designs
and fabrication techniques; and (v) commercial and portable electrochemical readouts. Together, this
review provides a comprehensive overview of the current advances in the design, fabrication and
application of optical and electrochemical O2 sensors.
Description
Keywords
oxygen sensors, microfluidics, organ-on-chips (OOCs), on-chip monitoring
Citation
Azimzadeh, M., Khashavar, P., Amereh, M., Tasnim, N., Hoorfar, M., & Akbari, M. (2021). “Microfluidic-based oxygen (O2) sensors for on-chip monitoring of cell, tissue and organ metabolism.” Biosensors, 12(1), 6. https://doi.org/10.3390/bios12010006