Transcriptomics and Proteomics Reveal the Cellulose and Pectin Metabolic Processes in the Tension Wood (Non-G-Layer) of Catalpa bungei

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dc.contributor.authorXiao, Yao
dc.contributor.authorYi, Fei
dc.contributor.authorLing, Juanjuan
dc.contributor.authorWang, Zhi
dc.contributor.authorZhao, Kun
dc.contributor.authorLu, Nan
dc.contributor.authorQu, Guanzheng
dc.contributor.authorKong, Lisheng
dc.contributor.authorMa, Wenjun
dc.contributor.authorWang, Junhui
dc.date.accessioned2020-03-09T14:14:37Z
dc.date.available2020-03-09T14:14:37Z
dc.date.copyright2020en_US
dc.date.issued2020
dc.description.abstractCatalpa bungei is an economically important tree with high-quality wood and highly valuable to the study of wood formation. In this work, the xylem microstructure of C. bungei tension wood (TW) was observed, and we performed transcriptomics, proteomics and Raman spectroscopy of TW, opposite wood (OW) and normal wood (NW). The results showed that there was no obvious gelatinous layer (G-layer) in the TW of C. bungei and that the secondary wall deposition in the TW was reduced compared with that in the OW and NW. We found that most of the differentially expressed mRNAs and proteins were involved in carbohydrate polysaccharide synthesis. Raman spectroscopy results indicated that the cellulose and pectin content and pectin methylation in the TW were lower than those in the OW and NW, and many genes and proteins involved in the metabolic pathways of cellulose and pectin, such as galacturonosyltransferase (GAUT), polygalacturonase (PG), endoglucanase (CLE) and β-glucosidase (BGLU) genes, were significantly upregulated in TW. In addition, we found that the MYB2 transcription factor may regulate the pectin degradation genes PG1 and PG3, and ARF, ERF, SBP and MYB1 may be the key transcription factors regulating the synthesis and decomposition of cellulose. In contrast to previous studies on TW with a G-layer, our results revealed a change in metabolism in TW without a G-layer, and we inferred that the change in the pectin type, esterification and cellulose characteristics in the TW of C. bungei may contribute to high tensile stress. These results will enrich the understanding of the mechanism of TW formation.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis work was supported by the Fundamental Research Funds of Chinese Academy of Forestry (CAFYBB2017ZY002)en_US
dc.identifier.citationXiao, Y., Yi, F., Ling, J., Wang, Z., Zhao, K., Lu, N., Qu, G., Kong, L., Ma, W. & Wang, J. (2020). Transcriptomics and Proteomics Reveal the Cellulose and Pectin Metabolic Processes in the Tension Wood (Non-G-Layer) of Catalpa bungei. International Journal of Molecular Sciences, 21(5), 1686. https://doi.org/10.3390/ijms21051686en_US
dc.identifier.urihttps://doi.org/10.3390/ijms21051686
dc.identifier.urihttp://hdl.handle.net/1828/11610
dc.language.isoenen_US
dc.publisherInternational Journal of Molecular Scienceen_US
dc.subjecttension wooden_US
dc.subjecttranscriptomeen_US
dc.subjectproteomicsen_US
dc.subjectRaman spectroscopyen_US
dc.subjectcelluloseen_US
dc.subjectpectinen_US
dc.subjectCatalpa bungeien_US
dc.titleTranscriptomics and Proteomics Reveal the Cellulose and Pectin Metabolic Processes in the Tension Wood (Non-G-Layer) of Catalpa bungeien_US
dc.typeArticleen_US

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