In-depth proteome coverage of in vitro-cultured Treponema pallidum and quantitative comparison analyses with in vivo-grown treponemes

Files

houston_jProteomeRes_2024.pdf (4.76 MB)

Date

2024

Authors

Houston, Simon
Gomez, Alloysius
Geppert, Andrew
Goodyear, Mara C.
Cameron, Caroline E.

Journal Title

Journal ISSN

Volume Title

Publisher

Journal of Proteome Research

Abstract

Previous mass spectrometry (MS)-based global proteomics studies have detected a combined total of 86% of all Treponema pallidum proteins under infection conditions (in vivo-grown T. pallidum). Recently, a method was developed for the long-term culture of T. pallidum under in vitro conditions (in vitro-cultured T. pallidum). Herein, we used our previously reported optimized MS-based proteomics approach to characterize the T. pallidum global protein expression profile under in vitro culture conditions. These analyses provided a proteome coverage of 94%, which extends the combined T. pallidum proteome coverage from the previously reported 86% to a new combined total of 95%. This study provides a more complete understanding of the protein repertoire of T. pallidum. Further, comparison of the in vitro-expressed proteome with the previously determined in vivo-expressed proteome identifies only a few proteomic changes between the two growth conditions, reinforcing the suitability of in vitro-cultured T. pallidum as an alternative to rabbit-based treponemal growth. The MS proteomics data have been deposited in the MassIVE repository with the data set identifier MSV000093603 (ProteomeXchange identifier PXD047625).

Description

Keywords

Treponema pallidum, syphilis, global proteomics, mass spectrometry (MS), protein expression profile, outer membrane proteins, vaccine candidates, Sexual and Reproductive Health and Rights (SRHR) Aspiration Research Cluster

Citation

Houston, S., Gomez, A., Geppert, A., Goodyear, M. C., & Cameron, C. E. (2024). In-depth proteome coverage of in vitro-cultured Treponema pallidum and quantitative comparison analyses with in vivo-grown treponemes. Journal of Proteome Research, 23(5), 1725–1743. https://doi.org/10.1021/acs.jproteome.3c00891

URI

https://doi.org/10.1021/acs.jproteome.3c00891
 https://hdl.handle.net/1828/23856

Collections

SRHR Aspiration Research Cluster Publications
 
University of Victoria Libraries

We acknowledge and respect the Lək̓ʷəŋən (Songhees and Esquimalt) Peoples on whose territory the university stands, and the Lək̓ʷəŋən and W̱SÁNEĆ Peoples whose historical relationships with the land continue to this day.