In-depth proteome coverage of in vitro-cultured Treponema pallidum and quantitative comparison analyses with in vivo-grown treponemes

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dc.contributor.authorHouston, Simon
dc.contributor.authorGomez, Alloysius
dc.contributor.authorGeppert, Andrew
dc.contributor.authorGoodyear, Mara C.
dc.contributor.authorCameron, Caroline E.
dc.date.accessioned2026-05-07T17:31:20Z
dc.date.available2026-05-07T17:31:20Z
dc.date.issued2024
dc.description.abstractPrevious mass spectrometry (MS)-based global proteomics studies have detected a combined total of 86% of all Treponema pallidum proteins under infection conditions (in vivo-grown T. pallidum). Recently, a method was developed for the long-term culture of T. pallidum under in vitro conditions (in vitro-cultured T. pallidum). Herein, we used our previously reported optimized MS-based proteomics approach to characterize the T. pallidum global protein expression profile under in vitro culture conditions. These analyses provided a proteome coverage of 94%, which extends the combined T. pallidum proteome coverage from the previously reported 86% to a new combined total of 95%. This study provides a more complete understanding of the protein repertoire of T. pallidum. Further, comparison of the in vitro-expressed proteome with the previously determined in vivo-expressed proteome identifies only a few proteomic changes between the two growth conditions, reinforcing the suitability of in vitro-cultured T. pallidum as an alternative to rabbit-based treponemal growth. The MS proteomics data have been deposited in the MassIVE repository with the data set identifier MSV000093603 (ProteomeXchange identifier PXD047625).
dc.description.reviewstatusReviewed
dc.description.scholarlevelFaculty
dc.identifier.citationHouston, S., Gomez, A., Geppert, A., Goodyear, M. C., & Cameron, C. E. (2024). In-depth proteome coverage of in vitro-cultured Treponema pallidum and quantitative comparison analyses with in vivo-grown treponemes. Journal of Proteome Research, 23(5), 1725–1743. https://doi.org/10.1021/acs.jproteome.3c00891
dc.identifier.urihttps://doi.org/10.1021/acs.jproteome.3c00891
dc.identifier.urihttps://hdl.handle.net/1828/23856
dc.language.isoen
dc.publisherJournal of Proteome Research
dc.rightsCC BY-NC-ND
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectTreponema pallidum
dc.subjectsyphilis
dc.subjectglobal proteomics
dc.subjectmass spectrometry (MS)
dc.subjectprotein expression profile
dc.subjectouter membrane proteins
dc.subjectvaccine candidates
dc.subjectSexual and Reproductive Health and Rights (SRHR) Aspiration Research Cluster
dc.subject.departmentDepartment of Biochemistry and Microbiology
dc.titleIn-depth proteome coverage of in vitro-cultured Treponema pallidum and quantitative comparison analyses with in vivo-grown treponemes
dc.typeArticle

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