Mammalian HP1 Isoforms Have Specific Roles in Heterochromatin Structure and Organization

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dc.contributor.authorBosch-Presegué, Laia
dc.contributor.authorRaurell-Vila, Helena
dc.contributor.authorThackray, Joshua K.
dc.contributor.authorGonzález, Jessica
dc.contributor.authorCasal, Carmen
dc.contributor.authorKane-Goldsmith, Noriko
dc.contributor.authorVizoso, Miguel
dc.contributor.authorBrown, Jeremy P.
dc.contributor.authorAntonio, Gómez
dc.contributor.authorAusió, Juan
dc.contributor.authorZimmermann, Timo
dc.contributor.authorEsteller, Manel
dc.contributor.authorSchotta, Gunnar
dc.contributor.authorSingh, Prim B.
dc.contributor.authorSerrano, Lourdes
dc.contributor.authorVaquero, Alejandro
dc.date.accessioned2019-03-21T00:14:02Z
dc.date.available2019-03-21T00:14:02Z
dc.date.copyright2017en_US
dc.date.issued2017
dc.description.abstractHP1 is a structural component of heterochromatin. Mammalian HP1 isoforms HP1 alpha, HP1 beta, and HP1 gamma play different roles in genome stability, but their precise role in heterochromatin structure is unclear. Analysis of Hp1 alpha(-/-), Hp1 beta(-/-), and Hp1 gamma(-/-) MEFs show that HP1 proteins have both redundant and unique functions within pericentric heterochromatin (PCH) and also act globally throughout the genome. HP1a confines H4K20me3 and H3K27me3 to regions within PCH, while its absence results in a global hyper-compaction of chromatin associated with a specific pattern of mitotic defects. In contrast, HP1b is functionally associated with Suv4-20h2 and H4K20me3, and its loss induces global chromatin decompaction and an abnormal enrichment of CTCF in PCH and other genomic regions. Our work provides insight into the roles of HP1 proteins in heterochromatin structure and genome stability.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipWe thank the members of the Vaquero laboratory, Dr. Karen Schindler, Dr. Sonia Guil, and Dr. Javier Rodriguez-Ubreva, for support and fruitful discussion; Raquel Garcia and the CRG Microscopy Unit for support on FLIM-FRET experiments; and David Lleres, Angus I. Lamond, Victor V. Lobanenkov, Mien-Chie Hung, Alan Underhill, Peter Hemmerich, Chris Wilson, and Masayuki Sekimata for sharing reagents. This work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO) (SAF2011-25860 and SAF2014-55964R to A.V.) and cofunded by FEDER funds/European Regional Development Fund (ERDF)-a way to build Europe, the Catalan Government Agency AGAUR (2009SGR-914 and 2014SGR-400 to A.V.), HGINJ (to L.S.), Deutsche Forschungsgemeinschaft (SFB1064 to G.S.), and the Canadian Institutes of Health Research (CIHR) (MOP-97878 to J.A.).en_US
dc.identifier.citationBosch-Presegué, L.; Raurell-Vila, H.; Thackray, J. K.; González, J.; Casal, C.; Kane-Goldsmith, N.; … & Vaquero, A. (2017). Mammalian HP1 isoforms have specific roles in heterochromatin structure and organization. Cell Reports, 21(8), 2048- 2057. DOI: 10.1016/j.celrep.2017.10.092en_US
dc.identifier.urihttps://doi.org/10.1016/j.celrep.2017.10.092
dc.identifier.urihttp://hdl.handle.net/1828/10657
dc.language.isoenen_US
dc.publisherCell Reportsen_US
dc.subjectheterochromatin
dc.subjectHP1α
dc.subjectHP1β
dc.subjectHP1γ
dc.subjectSuv420h2
dc.subjectH4K20me3
dc.subjectgenome organization
dc.subjectgenome stability
dc.subjectCentre for Biomedical Research
dc.subject.departmentDepartment of Biochemistry and Microbiology
dc.titleMammalian HP1 Isoforms Have Specific Roles in Heterochromatin Structure and Organizationen_US
dc.typeArticleen_US

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