Droplet-Based Microfluidic Platform for High-Throughput, Multi-Parameter Screening of Photosensitizer Activity

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dc.contributor.authorCho, Soongwon
dc.contributor.authorKang, Dong-Ku
dc.contributor.authorSim, Steven
dc.contributor.authorGeier, Florian
dc.contributor.authorKim, Jin-Young
dc.contributor.authorNiu, Xize
dc.contributor.authorEdel, Joshua B.
dc.contributor.authorChang, Soo-Ik
dc.contributor.authorWootton, Robert C. R.
dc.contributor.authorElvira, Katherine S.
dc.contributor.authordeMello, Andrew J.
dc.date.accessioned2018-11-09T20:50:46Z
dc.date.available2018-11-09T20:50:46Z
dc.date.copyright2013en_US
dc.date.issued2013
dc.description.abstractWe present a fully integrated droplet-based microfluidic platform for the high-throughput assessment of photodynamic therapy photosensitizer (PDT) efficacy on Escherichia coli. The described platform is able to controllably encapsulate cells and photosensitizer within pL-volume droplets, incubate the droplets over the course of several days, add predetermined concentrations of viability assay agents, expose droplets to varying doses of electromagnetic radiation, and detect both live and dead cells online to score cell viability. The viability of cells after encapsulation and incubation is assessed in a direct fashion, and the viability scoring method is compared to model live/dead systems for calibration. Final results are validated against conventional colony forming unit assays. In addition, we show that the platform can be used to perform concurrent measurements of light and dark toxicity of the PDT agents and that the platform allows simultaneous measurement of experimental parameters that include dark toxicity, photosensitizer concentration, light dose, and oxygenation levels for the development and testing of PDT agents.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipWe acknowledge Mark Draper for electrode fabrication, Robert Jarvis for the initial work and Professor Mark Wainwright for providing sensitizers and valuable advice. This work was partially supported by the National Research Foundation of Korea (Global Research Laboratory Programme Grant K20904000004-10A0500-00410).en_US
dc.identifier.citationCho, S., Kang, D., Sim, S., Geier, F., Kim, J., Niu, X., Edel, J.B., Chang, S., Wootton, R.C.R., Elvira, K.S. & deMello, A.J. (2013). Droplet-Based Microfluidic Platform for High-Throughput, Multi-Parameter Screening of Photosensitizer Activity. Analytical Chemistry, 85(18), 8866–8872. https://pubs.acs.org/doi/10.1021/ac4022067en_US
dc.identifier.urihttps://pubs.acs.org/doi/10.1021/ac4022067
dc.identifier.urihttp://hdl.handle.net/1828/10287
dc.language.isoenen_US
dc.publisherAnalytical Chemistryen_US
dc.subject.departmentDepartment of Chemistry
dc.titleDroplet-Based Microfluidic Platform for High-Throughput, Multi-Parameter Screening of Photosensitizer Activityen_US
dc.typePostprinten_US

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