Peptide biomarkers used for the selective breeding of a complex polygenic trait in honey bees
| dc.contributor.author | Guarna, M. Marta | |
| dc.contributor.author | Hoover, Shelley E. | |
| dc.contributor.author | Huxter, Elizabeth | |
| dc.contributor.author | Higo, Heather | |
| dc.contributor.author | Moon, Kyung-Mee | |
| dc.contributor.author | Domanski, Dominik | |
| dc.contributor.author | Bixby, Miriam E. F. | |
| dc.contributor.author | Melathopoulos, Andony P. | |
| dc.contributor.author | Ibrahim, Abdullah | |
| dc.contributor.author | Peirson, Michael | |
| dc.contributor.author | Desai, Suresh | |
| dc.contributor.author | Micholson, Derek | |
| dc.contributor.author | White, Rick | |
| dc.contributor.author | Borchers, Christoph H. | |
| dc.contributor.author | Currie, Robert W. | |
| dc.contributor.author | Pernal, Stephen F. | |
| dc.contributor.author | Foster, Leonard J. | |
| dc.date.accessioned | 2018-12-10T17:35:33Z | |
| dc.date.available | 2018-12-10T17:35:33Z | |
| dc.date.copyright | 2017 | en_US |
| dc.date.issued | 2017 | |
| dc.description.abstract | We present a novel way to select for highly polygenic traits. For millennia, humans have used observable phenotypes to selectively breed stronger or more productive livestock and crops. Selection on genotype, using single-nucleotide polymorphisms (SNPs) and genome profiling, is also now applied broadly in livestock breeding programs; however, selection on protein/peptide or mRNA expression markers has not yet been proven useful. Here we demonstrate the utility of protein markers to select for disease-resistant hygienic behavior in the European honey bee (Apis mellifera L.). Robust, mechanistically-linked protein expression markers, by integrating cis- and trans- effects from many genomic loci, may overcome limitations of genomic markers to allow for selection. After three generations of selection, the resulting marker-selected stock outperformed an unselected benchmark stock in terms of hygienic behavior, and had improved survival when challenged with a bacterial disease or a parasitic mite, similar to bees selected using a phenotype–based assessment for this trait. This is the first demonstration of the efficacy of protein markers for industrial selective breeding in any agricultural species, plant or animal. | en_US |
| dc.description.reviewstatus | Reviewed | en_US |
| dc.description.scholarlevel | Faculty | en_US |
| dc.description.sponsorship | The authors wish to thank Susan Cobey for assistance with instrumental insemination, Derek Smith for assistance with MRM assay development, and Immacolata Iovinella and Paolo Pelosi for providing recombinant Odorant Protein 16 for MRM peptide selection. We also thank Lisa Babey, Zoe Rempel, Rasoul Bahreini, Lindsay Geisel, Carl To, Jaclyn Deonarine, Daryl Wright, Cole Robson-Hyska, Sarah Carson, Dave Holder, and Lynae Ovinge for technical assistance. We sincerely thank each cooperating beekeeper that facilitated hygienic behavior testing in their operations and donated queens to the project. This work was supported by funding from Genome Canada and Genome BC through the BeeIPM project (107BEE). We would like to thank Genome Canada and Genome British Columbia for providing support to the University of Victoria-Genome British Columbia Proteomics Centre and the University of British Columbia through the Genome Innovations Network (204PRO for operations; 214PRO for technology development). C.H.B is also grateful for support from the Leading Edge Endowment Fund. and the Segal McGill Chair in Molecular Oncology at McGill University (Montreal, Quebec, Canada). C.H.B. is also grateful for support from the Warren Y. Soper Charitable Trust and the Alvin Segal Family Foundation to the Jewish General Hospital (Montreal, Quebec, Canada). | en_US |
| dc.identifier.citation | Guarna, M.M.; Hoover, S.E.; Huxter, E.; Higo, H.; Moon, K.; Domanski, D.; … & Foster, L.J. (2017). Peptide biomarkers used for the selective breeding of a complex polygenic trait in honey bees. Scientific Reports, 7, article 8381. DOI: 10.1038/s41598-017-08464-2 | en_US |
| dc.identifier.uri | https://doi.org/10.1038/s41598-017-08464-2 | |
| dc.identifier.uri | http://hdl.handle.net/1828/10400 | |
| dc.language.iso | en | en_US |
| dc.publisher | Scientific Reports | en_US |
| dc.subject | UVic Genome BC Proteomics Centre | |
| dc.subject.department | Department of Microbiology and Biochemistry | |
| dc.subject.department | Department of Biochemistry and Microbiology | |
| dc.title | Peptide biomarkers used for the selective breeding of a complex polygenic trait in honey bees | en_US |
| dc.type | Article | en_US |