Long-term successional subculture dynamics and their effects on the proliferation efficiency, embryogenic potential, and genetic stability of embryogenic tissues in Larix principis-rupprechtii Mayr
Date
2024
Authors
Chen, Xiaoyi
Liu, Chengbi
Yuan, Deshui
Wang, Xiuqi
Zhao, Huanhuan
Zhang, Luyao
Kong, Lisheng
Zhang, Jinfeng
Zhao, Jian
Journal Title
Journal ISSN
Volume Title
Publisher
Forests
Abstract
Larix principis-rupprechtii Mayr, a coniferous species indigenous to Northern China, possesses significant ecological and economic value. Somatic embryogenesis offers a pathway with significant potential for large-scale propagation, long-term germplasm conservation, and genetic transformation in L. principis-rupprechtii Mayr. However, it remains unclear whether significant variations occur in embryogenic tissues during long-term successive subculturing, which could impact the productivity of somatic embryos. This is a pivotal concern that lacks comprehensive understanding. In this study, three embryogenic cell lines were used to explore the dynamics and relationships among proliferation rate, pre-treatment proliferation rate, and embryogenic capabilities across a series of 32 subculturing cycles. Proliferation rate, pre-treatment proliferation rate, and somatic embryo maturation rate showed no significant correlation with subculturing cycles. However, there was a positive correlation between subculturing cycles and pre-treatment proliferation rate and a negative correlation with somatic embryo maturation rate in the BFU1 cell line. In addition, we utilized ten SSR molecular markers to investigate the genetic stability in embryogenic tissues during long-term subculturing. No genomic variations were detected in any of the three embryogenic cell lines, which suggests that the observed phenotypic dynamics during subculturing may not be primarily driven by genomic alterations. This study provides novel insights into the dynamics of the long-term culture of embryogenic tissues, laying a foundation for the optimization and application of somatic embryogenesis techniques in L. principis-rupprechtii Mayr and potentially other coniferous species.
Description
Keywords
conifer, genetic stability, larch, mature capacity, proliferation efficiency, somatic embryos, SSR molecular markers, Centre for Forest Biology
Citation
Chen, X., Liu, C., Yuan, D., Wang, X., Zhao, H., Zhang, L., Kong, L., Zhang, J., & Zhao, J. (2024). Long-term successional subculture dynamics and their effects on the proliferation efficiency, embryogenic potential, and genetic stability of embryogenic tissues in Larix principis-rupprechtii Mayr. Forests, 15(4), Article 4. https://doi.org/10.3390/f15040627