An Engineered Infected Epidermis Model for In Vitro Study of the Skin’s Pro- Inflammatory Response

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dc.contributor.authorJahanshahi, Maryam
dc.contributor.authorHamdi, David
dc.contributor.authorGodau, Brent
dc.contributor.authorSamiei, Ehsan
dc.contributor.authorSanchez- Lafuente, Carla Liria
dc.contributor.authorNeale, Katie J.
dc.contributor.authorHadisi, Zhina
dc.contributor.authorDabiri, Seyed Mohammad Hossein
dc.contributor.authorPagan, Erik
dc.contributor.authorChristie, Brian R.
dc.contributor.authorAkbari, Mohsen
dc.date.accessioned2023-11-30T17:47:09Z
dc.date.available2023-11-30T17:47:09Z
dc.date.copyright2020en_US
dc.date.issued2020-02
dc.description.abstractWound infection is a major clinical challenge that can significantly delay the healing process, can create pain, and requires prolonged hospital stays. Pre-clinical research to evaluate new drugs normally involves animals. However, ethical concerns, cost, and the challenges associated with interspecies variation remain major obstacles. Tissue engineering enables the development of in vitro human skin models for drug testing. However, existing engineered skin models are representative of healthy human skin and its normal functions. This paper presents a functional infected epidermis model that consists of a multilayer epidermis structure formed at an air-liquid interface on a hydrogel matrix and a three-dimensionally (3D) printed vascular-like network. The function of the engineered epidermis is evaluated by the expression of the terminal differentiation marker, filaggrin, and the barrier function of the epidermis model using the electrical resistance and permeability across the epidermal layer. The results showed that the multilayer structure enhances the electrical resistance by 40% and decreased the drug permeation by 16.9% in the epidermis model compared to the monolayer cell culture on gelatin. We infect the model with Escherichia coli to study the inflammatory response of keratinocytes by measuring the expression level of pro-inflammatory cytokines (interleukin 1 beta and tumor necrosis factor alpha). After 24 h of exposure to Escherichia coli, the level of IL-1β and TNF-α in control samples were 125 ± 78 and 920 ± 187 pg/mL respectively, while in infected samples, they were 1429 ± 101 and 2155.5 ± 279 pg/mL respectively. However, in ciprofloxacin-treated samples the levels of IL-1β and TNF-α without significant difference with respect to the control reached to 246 ± 87 and 1141.5 ± 97 pg/mL respectively. The robust fabrication procedure and functionality of this model suggest that the model has great potential for modeling wound infections and drug testing.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis research was funded by the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant, the Canada Foundation for Innovation (CFI), and the Canadian Institutes for Health Research (CIHR).en_US
dc.identifier.citationJahanshahi, M., Hamdi, D., Godau, B., Samiei, E., Sanchez-Lafuente, C.L., Neale, K.J., … Akbari, M. (2020). An engineered infected epidermis model for In vitro study of the skin’s pro-inflammatory response. Micromachines, 11(2), 227. https://doi.org/10.3390/mi11020227en_US
dc.identifier.urihttp://dx.doi.org/10.3390/mi11020227
dc.identifier.urihttp://hdl.handle.net/1828/15665
dc.language.isoenen_US
dc.publisherMicromachinesen_US
dc.subjectepidermis
dc.subject3D bioprinting
dc.subjectwound modeling
dc.subjectinfection
dc.subjectpro-inflammatory response
dc.subjectLaboratory for Innovations in Micro Engineering (LiME)
dc.subject.departmentDivision of Medical Sciences
dc.subject.departmentSchool of Medical Sciences
dc.subject.departmentDepartment of Mechanical Engineering
dc.titleAn Engineered Infected Epidermis Model for In Vitro Study of the Skin’s Pro- Inflammatory Responseen_US
dc.typeArticleen_US

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