Affinity Purification Coupled to Stable Isotope Dilution LC-MS/MS Analysis to Discover IgG4 Glycosylation Profiles for Autoimmune Pancreatitis
Date
2021
Authors
Chen, Michael X.
Su, Ho-Hsuan
Shiao, Ching-Ya
Chang, Yu-Ting
Chang, Ming-Chu
Kao, Chih-Chin
Wang, San-Yuan
Shih, His-Chang
Tsai, I-Lin
Journal Title
Journal ISSN
Volume Title
Publisher
International Journal of Molecular Sciences
Abstract
Type 1 autoimmune pancreatitis (AIP) is categorized as an IgG4-related disease (IgG4-RD),
where a high concentration of plasma IgG4 is one of the common biomarkers among patients. IgG
Fc-glycosylation has been reported to be potential biosignatures for diseases. However, human
IgG3 and IgG4 Fc-glycopeptides from populations in Asia were found to be isobaric ions when
using LC-MS/MS as an analytical tool. In this study, an analytical workflow that coupled affinity
purification and stable isotope dilution LC-MS/MS was developed to dissect IgG4 glycosylation
profiles for autoimmune pancreatitis. Comparing the IgG4 and glycosylation profiles among healthy
controls, patients with pancreatic ductal adenocarcinoma (PDAC), and AIP, the IgG4 glycosylations
from the AIP group were found to have more digalactosylation (compared to PDAC) and less
monogalactosylation (compared to HC). In addition, higher fucosylation and sialylation profiles were
also discovered for the AIP group. The workflow is efficient and selective for IgG4 glycopeptides,
and can be used for clinical biosignature discovery.
Description
We thank the technical support provided by TMU Core Facility. We would like to acknowledge Chun-Chih Jared Liu and Yi-Ju Chen for their excellent technical support at TMU Core Facility.
Keywords
type 1 autoimmune pancreatitis, IgG4, N-glycosylation, mass spectrometry
Citation
Chen, M. X., Su, H.-H., Shiao, C.-Y., Chang, Y.-T., Chang, M.-C., Kao, C.-C., Wang, S.-Y., Shih, H.-C., & Tsai, I.-L. (2021). Affinity purification coupled to stable isotope dilution lc-ms/ms analysis to discover igg4 glycosylation profiles for autoimmune pancreatitis. International Journal of Molecular Sciences, 22(11527), 1-13. https://doi.org/10.3390/ijms222111527