Affinity Purification Coupled to Stable Isotope Dilution LC-MS/MS Analysis to Discover IgG4 Glycosylation Profiles for Autoimmune Pancreatitis

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dc.contributor.authorChen, Michael X.
dc.contributor.authorSu, Ho-Hsuan
dc.contributor.authorShiao, Ching-Ya
dc.contributor.authorChang, Yu-Ting
dc.contributor.authorChang, Ming-Chu
dc.contributor.authorKao, Chih-Chin
dc.contributor.authorWang, San-Yuan
dc.contributor.authorShih, His-Chang
dc.contributor.authorTsai, I-Lin
dc.date.accessioned2021-11-01T17:48:41Z
dc.date.available2021-11-01T17:48:41Z
dc.date.copyright2021en_US
dc.date.issued2021
dc.descriptionWe thank the technical support provided by TMU Core Facility. We would like to acknowledge Chun-Chih Jared Liu and Yi-Ju Chen for their excellent technical support at TMU Core Facility.en_US
dc.description.abstractType 1 autoimmune pancreatitis (AIP) is categorized as an IgG4-related disease (IgG4-RD), where a high concentration of plasma IgG4 is one of the common biomarkers among patients. IgG Fc-glycosylation has been reported to be potential biosignatures for diseases. However, human IgG3 and IgG4 Fc-glycopeptides from populations in Asia were found to be isobaric ions when using LC-MS/MS as an analytical tool. In this study, an analytical workflow that coupled affinity purification and stable isotope dilution LC-MS/MS was developed to dissect IgG4 glycosylation profiles for autoimmune pancreatitis. Comparing the IgG4 and glycosylation profiles among healthy controls, patients with pancreatic ductal adenocarcinoma (PDAC), and AIP, the IgG4 glycosylations from the AIP group were found to have more digalactosylation (compared to PDAC) and less monogalactosylation (compared to HC). In addition, higher fucosylation and sialylation profiles were also discovered for the AIP group. The workflow is efficient and selective for IgG4 glycopeptides, and can be used for clinical biosignature discovery.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis research and APC was funded by the Ministry of Science and Technology, Taiwan, grant number 107-2314-B-038-019-MY3 and 108-2320-B-038-060-MY3.en_US
dc.identifier.citationChen, M. X., Su, H.-H., Shiao, C.-Y., Chang, Y.-T., Chang, M.-C., Kao, C.-C., Wang, S.-Y., Shih, H.-C., & Tsai, I.-L. (2021). Affinity purification coupled to stable isotope dilution lc-ms/ms analysis to discover igg4 glycosylation profiles for autoimmune pancreatitis. International Journal of Molecular Sciences, 22(11527), 1-13. https://doi.org/10.3390/ijms222111527en_US
dc.identifier.urihttps://doi.org/10.3390/ijms222111527
dc.identifier.urihttp://hdl.handle.net/1828/13472
dc.language.isoenen_US
dc.publisherInternational Journal of Molecular Sciencesen_US
dc.subjecttype 1 autoimmune pancreatitisen_US
dc.subjectIgG4en_US
dc.subjectN-glycosylationen_US
dc.subjectmass spectrometryen_US
dc.titleAffinity Purification Coupled to Stable Isotope Dilution LC-MS/MS Analysis to Discover IgG4 Glycosylation Profiles for Autoimmune Pancreatitisen_US
dc.typeArticleen_US

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