Modeling the behavior of human induced pluripotent stem cells seeded on melt electrospun scaffolds




Hall, Meghan E.
Mohtaram, Nima Khadem
Edwards, Roderick
Willeth, Stephanie

Journal Title

Journal ISSN

Volume Title


Journal of Biological Engineering


Background: Human induced pluripotent stem cells (hiPSCs) can form any tissue found in the body, making them attractive for regenerative medicine applications. Seeding hiPSC aggregates into biomaterial scaffolds can control their differentiation into specific tissue types. Here we develop and analyze a mathematical model of hiPSC aggregate behavior when seeded on melt electrospun scaffolds with defined topography. Results: We used ordinary differential equations to model the different cellular populations (stem, progenitor, differentiated) present in our scaffolds based on experimental results and published literature. Our model successfully captures qualitative features of the cellular dynamics observed experimentally. We determined the optimal parameter sets to maximize specific cellular populations experimentally, showing that a physiologic oxygen level (∼5%) increases the number of neural progenitors and differentiated neurons compared to atmospheric oxygen levels (∼21%) and a scaffold porosity of ∼63% maximizes aggregate size. Conclusions: Our mathematical model determined the key factors controlling hiPSC behavior on melt electrospun scaffolds, enabling optimization of experimental parameters.



Stem cell, Ordinary differential equation, Differentiation, Proliferation, Tissue engineering


Hall, M.E., Mohtaram, N.K., Willerth, S.M., & Edwards, R. (2017). Modeling the behaviour of human induced pluripotent stem cells seeded on melt electrospun scaffolds. Journal of Biological Engineering, 11(38).