Modeling the behavior of human induced pluripotent stem cells seeded on melt electrospun scaffolds

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dc.contributor.authorHall, Meghan E.
dc.contributor.authorMohtaram, Nima Khadem
dc.contributor.authorEdwards, Roderick
dc.contributor.authorWilleth, Stephanie
dc.date.accessioned2021-08-05T22:11:48Z
dc.date.available2021-08-05T22:11:48Z
dc.date.copyright2017en_US
dc.date.issued2017
dc.description.abstractBackground: Human induced pluripotent stem cells (hiPSCs) can form any tissue found in the body, making them attractive for regenerative medicine applications. Seeding hiPSC aggregates into biomaterial scaffolds can control their differentiation into specific tissue types. Here we develop and analyze a mathematical model of hiPSC aggregate behavior when seeded on melt electrospun scaffolds with defined topography. Results: We used ordinary differential equations to model the different cellular populations (stem, progenitor, differentiated) present in our scaffolds based on experimental results and published literature. Our model successfully captures qualitative features of the cellular dynamics observed experimentally. We determined the optimal parameter sets to maximize specific cellular populations experimentally, showing that a physiologic oxygen level (∼5%) increases the number of neural progenitors and differentiated neurons compared to atmospheric oxygen levels (∼21%) and a scaffold porosity of ∼63% maximizes aggregate size. Conclusions: Our mathematical model determined the key factors controlling hiPSC behavior on melt electrospun scaffolds, enabling optimization of experimental parameters.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThe study was supported by Natural Sciences and Engineering Research Council (NSERC) Discovery Grants.en_US
dc.identifier.citationHall, M.E., Mohtaram, N.K., Willerth, S.M., & Edwards, R. (2017). Modeling the behaviour of human induced pluripotent stem cells seeded on melt electrospun scaffolds. Journal of Biological Engineering, 11(38). https://doi.org/10.1186/s13036-017-0080-5en_US
dc.identifier.urihttps://doi.org/10.1186/s13036-017-0080-5
dc.identifier.urihttp://hdl.handle.net/1828/13208
dc.language.isoenen_US
dc.publisherJournal of Biological Engineeringen_US
dc.subjectStem cell
dc.subjectOrdinary differential equation
dc.subjectDifferentiation
dc.subjectProliferation
dc.subjectTissue engineering
dc.subjectCentre for Biomedical Research
dc.subject.departmentDepartment of Mathematics and Statistics
dc.subject.departmentDepartment of Mechanical Engineering
dc.subject.departmentDivision of Medical Sciences
dc.subject.departmentSchool of Medical Sciences
dc.titleModeling the behavior of human induced pluripotent stem cells seeded on melt electrospun scaffoldsen_US
dc.typeArticleen_US

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