A Comprehensive Survey of the Genes Involved in Maturation and Development of the Rainbow Trout Ovary

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dc.contributor.authorvon Schalburg, Kristian R.
dc.contributor.authorWise, Matthew L.
dc.contributor.authorBrown, Gordon D.
dc.contributor.authorDavidson, William S.
dc.contributor.authorKoop, Ben F.
dc.date.accessioned2015-08-11T19:53:14Z
dc.date.available2015-08-11T19:53:14Z
dc.date.copyright2005en_US
dc.date.issued2005-10-20
dc.descriptionPost-printen_US
dc.description.abstractThe development and maturation of the ovary requires precisely coordinated expression of specific gene-classes to produce viable oocytes. We undertook identification of some of the genes 26 involved in these processes by creating ovary-specific cDNA libraries by suppression subtractive hybridization and by microarray-based analyses. We present 5778 tissue- and sex-specific genes from subtracted ovary and testis libraries, many of which remain unidentified. A microarray containing 3557 salmonid cDNAs was used to compare the transcriptomes of precocious ovary at three different stages during second year with a reference (normal ovary) transcriptome. On average, about 240 genes were developmentally regulated during the study period from June to October. Classes of genes maintaining relatively steady-state levels of expression, such as those controlling tissue remodeling, immunoregulation, cell-cycle progression, apoptosis and growth were also identified. Concurrent expression of various cell division and ubiquitin-mediated proteolysis regulators revealed the utility of microarray analysis to monitor important maturation events. We also report unequivocal evidence for expression of the transcripts that encode the common glycoprotein-α (Cgα), LHβ, FSHβ and TSHβ subunits, and retinol-binding protein in both the ovary and testis of trout.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipNSERC; Genome Canada; Genome B.C.en_US
dc.identifier.citationKristian R. von Schalburg, Rise, M.L., Brown, G.D., Davidson, W.S. & Koop, B.F. 2005, "A Comprehensive Survey of the Genes Involved in Maturation and Development of the Rainbow Trout Ovary", Biology of reproduction, vol. 72, no. 3, pp. 687-699.en_US
dc.identifier.urihttp://dx.doi.org/10.1095/biolreprod.104.034967
dc.identifier.urihttp://hdl.handle.net/1828/6421
dc.language.isoenen_US
dc.publisherSociety for the Study of Reproductionen_US
dc.subjectovary
dc.subjecttestis
dc.subjectcDNA
dc.subjectmicroarray
dc.subjectrainbow trout
dc.subjectCentre for Biomedical Research
dc.subject.departmentDepartment of Biology
dc.titleA Comprehensive Survey of the Genes Involved in Maturation and Development of the Rainbow Trout Ovaryen_US
dc.typeArticleen_US

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