Structure–activity correlation in transfection promoted by pyridinium cationic lipids

Date

2016

Authors

Parvizi-Bahktar, P.
Mendez-Campos, J.
Raju, L.
Khalique, N. A.
Jubeli, E.
Larsen, H.
Nicholson, D.
Pungente, M. D.
Fyles, Thomas M.

Journal Title

Journal ISSN

Volume Title

Publisher

Organic & Biomolecular Chemistry

Abstract

The efficiency of the transfection of a plasmid DNA encoding a galactosidase promoted by a series of pyridinium lipids in mixtures with other cationic lipids and neutral lipids was assessed in CHO-K1 cells. We identify key molecular parameters of the lipids in the mixture – clog P, lipid length, partial molar volume – to predict the morphology of the lipid-DNA lipoplex and then correlate these same parameters with transfection efficiency in an in vitro assay. We define a Transfection Index that provides a linear correlation with normalized transfection efficiency over a series of 90 different lipoplex compositions. We also explore the influence of the same set of molecular parameters on the cytotoxicity of the formulations.

Description

Keywords

Citation

Parvizi-Bahktar, P., Mendez-Campos, J., Raju, L., Khalique, N. A., Jubeli, E., Larsen, H., … Fyles, T. M. (2016). Structure–activity correlation in transfection promoted by pyridinium cationic lipids. Organic & Biomolecular Chemistry, 14(11), 3080-3090. https://doi.org/10.1039/c6ob00041j