Structure–activity correlation in transfection promoted by pyridinium cationic lipids

Simple item page
dc.contributor.authorParvizi-Bahktar, P.
dc.contributor.authorMendez-Campos, J.
dc.contributor.authorRaju, L.
dc.contributor.authorKhalique, N. A.
dc.contributor.authorJubeli, E.
dc.contributor.authorLarsen, H.
dc.contributor.authorNicholson, D.
dc.contributor.authorPungente, M. D.
dc.contributor.authorFyles, Thomas M.
dc.date.accessioned2020-11-03T23:08:26Z
dc.date.available2020-11-03T23:08:26Z
dc.date.copyright2016en_US
dc.date.issued2016
dc.description.abstractThe efficiency of the transfection of a plasmid DNA encoding a galactosidase promoted by a series of pyridinium lipids in mixtures with other cationic lipids and neutral lipids was assessed in CHO-K1 cells. We identify key molecular parameters of the lipids in the mixture – clog P, lipid length, partial molar volume – to predict the morphology of the lipid-DNA lipoplex and then correlate these same parameters with transfection efficiency in an in vitro assay. We define a Transfection Index that provides a linear correlation with normalized transfection efficiency over a series of 90 different lipoplex compositions. We also explore the influence of the same set of molecular parameters on the cytotoxicity of the formulations.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis work was made possible by a grant from the Qatar National Priorities Research Program, award NPRP08-705-3-144. The contents are solely the responsibility of the authors and do not necessarily represent the official views of the Qatar National Research Fund. Synchrotron beam time on the BioSAXS beam line (BM29) was provided by the European Synchrotron Radiation Facility in Grenoble, France. On-going support from the National Science and Engineering Research Council of Canada is gratefully acknowledged.en_US
dc.identifier.citationParvizi-Bahktar, P., Mendez-Campos, J., Raju, L., Khalique, N. A., Jubeli, E., Larsen, H., … Fyles, T. M. (2016). Structure–activity correlation in transfection promoted by pyridinium cationic lipids. Organic & Biomolecular Chemistry, 14(11), 3080-3090. https://doi.org/10.1039/c6ob00041jen_US
dc.identifier.urihttps://doi.org/10.1039/c6ob00041j
dc.identifier.urihttp://hdl.handle.net/1828/12306
dc.language.isoenen_US
dc.publisherOrganic & Biomolecular Chemistryen_US
dc.subject.departmentDepartment of Chemistry
dc.titleStructure–activity correlation in transfection promoted by pyridinium cationic lipidsen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Parvizi-Bahktar_P_OrgBiomolChem_2016.pdf
Size:
1.63 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Faculty and Staff Publications
 
University of Victoria Libraries

We acknowledge and respect the Lək̓ʷəŋən (Songhees and Esquimalt) Peoples on whose territory the university stands, and the Lək̓ʷəŋən and W̱SÁNEĆ Peoples whose historical relationships with the land continue to this day.