Conditional protein splicing of a-sarcin in live cells
Date
2014
Authors
Alford, Spencer C.
O’Sullivan, Connor
Obst, Jon
Christie, Jennifer
Howard, Perry L.
Journal Title
Journal ISSN
Volume Title
Publisher
Molecular BioSystems
Abstract
Protein splicing technology harnesses the ability of inteins to ligate protein fragments, forming a mature protein. This report describes our effort to engineer rapamycin-dependent protein splicing of a ribotoxin, called α-sarcin. Engineering this system required the investigation of important splicing parameters, including extein context and splicing temperature. We show α-sarcin splicing is dependent on rapamycin, is inducible with rapid kinetics, and triggers apoptosis in HeLa cells. These findings establish a proof-of-concept for a conditional cell ablation strategy.
Description
Keywords
Centre for Biomedical Research
Citation
Alford, S. C., Christie, J., Howard, P. L., Obst, J., & O’Sullivan, C. (2014). Conditional protein splicing of a-sarcin in live cells. Molecular BioSystems, 10(4), 831-837. https://doi.org/10.1039/c3mb70387h