Conditional protein splicing of a-sarcin in live cells

Date

2014

Authors

Alford, Spencer C.
O’Sullivan, Connor
Obst, Jon
Christie, Jennifer
Howard, Perry L.

Journal Title

Journal ISSN

Volume Title

Publisher

Molecular BioSystems

Abstract

Protein splicing technology harnesses the ability of inteins to ligate protein fragments, forming a mature protein. This report describes our effort to engineer rapamycin-dependent protein splicing of a ribotoxin, called α-sarcin. Engineering this system required the investigation of important splicing parameters, including extein context and splicing temperature. We show α-sarcin splicing is dependent on rapamycin, is inducible with rapid kinetics, and triggers apoptosis in HeLa cells. These findings establish a proof-of-concept for a conditional cell ablation strategy.

Description

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Citation

Alford, S. C., Christie, J., Howard, P. L., Obst, J., & O’Sullivan, C. (2014). Conditional protein splicing of a-sarcin in live cells. Molecular BioSystems, 10(4), 831-837. https://doi.org/10.1039/c3mb70387h