Conditional protein splicing of a-sarcin in live cells

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dc.contributor.authorAlford, Spencer C.
dc.contributor.authorO’Sullivan, Connor
dc.contributor.authorObst, Jon
dc.contributor.authorChristie, Jennifer
dc.contributor.authorHoward, Perry L.
dc.date.accessioned2020-10-09T18:38:29Z
dc.date.available2020-10-09T18:38:29Z
dc.date.copyright2014en_US
dc.date.issued2014
dc.description.abstractProtein splicing technology harnesses the ability of inteins to ligate protein fragments, forming a mature protein. This report describes our effort to engineer rapamycin-dependent protein splicing of a ribotoxin, called α-sarcin. Engineering this system required the investigation of important splicing parameters, including extein context and splicing temperature. We show α-sarcin splicing is dependent on rapamycin, is inducible with rapid kinetics, and triggers apoptosis in HeLa cells. These findings establish a proof-of-concept for a conditional cell ablation strategy.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThe authors thank Dr T. Muir for providing constructs containing the VMA intein fragments conjugated to the FRB and FKBP. This work was supported by a NSERC Discovery Grant awarded to PLH.en_US
dc.identifier.citationAlford, S. C., Christie, J., Howard, P. L., Obst, J., & O’Sullivan, C. (2014). Conditional protein splicing of a-sarcin in live cells. Molecular BioSystems, 10(4), 831-837. https://doi.org/10.1039/c3mb70387hen_US
dc.identifier.urihttps://doi.org/10.1039/c3mb70387h
dc.identifier.urihttp://hdl.handle.net/1828/12193
dc.language.isoenen_US
dc.publisherMolecular BioSystemsen_US
dc.subjectCentre for Biomedical Research
dc.subject.departmentDepartment of Biochemistry and Microbiology
dc.subject.departmentDepartment of Biology
dc.titleConditional protein splicing of a-sarcin in live cellsen_US
dc.typeArticleen_US

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