Pressurized Sample Infusion
Date
2021-12-15
Authors
Thomas, Gilian T.
Donnecke, Sofia
Chagunda, Ian C.
McIndoe, J. Scott
Journal Title
Journal ISSN
Volume Title
Publisher
Chemistry-Methods
Abstract
Pressurized sample infusion (PSI) is a simple and effective means of continuously introducing a solution to an electrospray ionization mass spectrometry (ESI-MS) source. It allows for acquisition of real-time data in an air- and moisture-free environment and requires minimal additional infrastructure. It is applicable for use for any reaction in which one or more components are detectable by ESI-MS and for which time-course information is desired over a time scale of seconds to minutes. The strengths and weaknesses of the method are critically examined, and technique tips and tricks are provided to enable maximal effectiveness when employing this approach to continuous monitoring of complex reaction mixtures.
Description
Thanks to all of the students who
developed and refined the PSI technique over the years, especially
Krista Kobylianskii, Zohrab Ahmadi, Jingwei Luo, Rhonda Stoddard, Lars Yunker, Eric Janusson, Robin Theron, Anuj Joshi, and
Isaac Omari.
Keywords
mass spectrometry, on-line monitoring, pressurized sample infusion, reaction mechanisms, real-time monitoring
Citation
Thomas, G.T., Donnecke, S., Chagunda, I.C., & McIndoe, J. S. (2021). Chemistry Methods, 2(1), https://doi.org/10.1002/cmtd.202100068