Electrostatically promoted dynamic hybridization of glucans with cationic polythiophene




Fukuhara, Gaku
Imai, Mami
Ishida, Yuki
Kurohara, Hiroki
Yang, Cheng
Mori, Tadashi
Uyama, Hiroshi
Cornelia, Bohne
Inouea, Yoshihisa
Fuentealba, Denis

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Organic & Biomolecular Chemistry


Hybridizing natural macromolecule with synthetic polymer is an efficient general method for constructing sophisticated supramolecular architectures. To comprehensively elucidate the controversial hybridization mechanism of glucans with synthetic polymers, the hybridization behaviors of triple-stranded curdlan (Cur) and schizophyllan (SPG) with cationic polythiophene (PyPT) were investigated in aqueous DMSO solutions by using UV-vis, circular dichroism (CD), fluorescence, fluorescence excitation, and NMR spectroscopies, as well as theoretical calculations, dynamic light scattering, and zeta potential measurements. Upon mixing with glucan, a hetero-triplex formed was dynamic and greatly accelerated by heating and by adding base or salt. The hetero-triplex disassembled to hetero-duplex in highly basic solutions. Thus, polycationic polymers, such as PyPT, are expected to serve as a versatile tool for unzipping glucan homo-triplexes and promoting subsequent hybridization in aqueous solution, while the detailed mechanism elucidated in the present study contributes to the rational design of hybridization partners.




Fukuhara, Gaku et al. (2016). Electrostatically promoted dynamic hybridization of glucans with cationic polythiophene. Organic & Biomolecular Chemistry, 14, 9741- 9750. doi 10.1039/C6OB01353H