Pannexin 1 Differentially Affects Neural Precursor Cell Maintenance in the Ventricular Zone and Peri-Infarct Cortex

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dc.contributor.authorWicki-Stordeur, Leigh E.
dc.contributor.authorSanchez-Arias, Juan C.
dc.contributor.authorDhaliwal, Jagroop
dc.contributor.authorCarmona-Wagner, Esther O.
dc.contributor.authorShestopalov, Valery I.
dc.contributor.authorLagace, Diane C.
dc.contributor.authorSwayne, Leigh Anne
dc.date.accessioned2016-04-12T06:34:25Z
dc.date.available2016-08-07T11:22:08Z
dc.date.copyright2016en_US
dc.date.issued2016-01
dc.descriptionSignificance Statement Here, we demonstrate that Pannexin 1 (Panx1) maintains a consistent population size of neural precursor cells in the ventricular zone, both in the healthy brain and in the context of stroke. In contrast, Panx1 appears to be detrimental to the survival of neural precursor cells that surround damaged cortical tissue in the stroke brain. This suggests that targeting Panx1 in the peri-infarct cortex, in combination with other therapies, could improve cell survival around the injury site.en_US
dc.description.abstractWe demonstrated previously that Pannexin 1 (Panx1), an ion and metabolite channel, promotes the growth and proliferation of ventricular zone (VZ) neural precursor cells (NPCs) in vitro.To investigate its role in vivo,we used floxed Panx1 mice in combination with viruses to delete Panx1 in VZ NPCs and to track numbers of Panx1-null and Panx1-expressing VZ NPCs over time. Two days after virus injection, Panx1-null cells were less abundant than Panx1-expressing cells, suggesting that Panx1 is required for the maintenance of VZ NPCs. We also investigated the effect of Panx1 deletion in VZ NPCs after focal cortical stroke via photothrombosis. Panx1 is essential for maintaining elevated VZ NPC numbers after stroke. In contrast, Panx1-null NPCs were more abundant than Panx1-expressing NPCs in the peri-infarct cortex. Together, these findings suggest that Panx1 plays an important role in NPC maintenance in the VZ niche in the naive and stroke brain and could be a key target for improving NPC survival in the peri-infarct cortex.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis work was supported by grants from the Heart and Stroke Foundation Canadian Partnership for Stroke Recovery (CPSR Expansion Grant to L.A.S. and D.C.L.), the Natural Sciences and Engineering Research Council of Canada (NSERC Discovery Grant to L.A.S.), the Canadian Institutes of Health Research (Grant MOP142215 to L.A.S.), and the University of Victoria Division of Medical Sciences (Seed Grant to L.A.S.). L.A.S. is supported by a Michael Smith Foundation for Health Research and a British Columbia Schizophrenia Society Foundation Scholar Award. L.E.W.-S. is supported by a Vanier Canada Graduate Scholarship (NSERC) and an Edythe Hembroff-Schleicher Scholarship. V.I.S. was supported by the National Eye Institute–National Institutes of Health (Grants EY021517, P30 EY014801, and RPB unrestricted Grant to the University of Miami Department of Ophthalmology). We thank Mirela Hasu, Angela Nguyen, Keren Leviel Kumar (University of Ottawa), and Anthony Carter (CPSR) for technical support and the Canadian Foundation for Innovation and the British Columbia Knowledge Development Fund for granting funds to L.A.S. for our Leica SP8 confocal microscope.en_US
dc.identifier.citationWicki-Stordeur, L.E., Sanchez-Arias, J.C., Dhaliwal, J., Carmona-Wagner, E.O., Shestopalov, V.I., Lagace, D.C. & Swayne, L.A. (2016). Pannexin 1 differentially affects neural precursor cell maintenance in the ventricular zone and peri-infarct cortex. The Journal of Neuroscience, 36(4), 1203-1210.en_US
dc.identifier.urihttp://dx.doi.org/10.1523/JNEUROSCI.0436-15.2016
dc.identifier.urihttp://hdl.handle.net/1828/7103
dc.language.isoenen_US
dc.publisherThe Journal of Neuroscienceen_US
dc.rightsAttribution 2.5 Canada*
dc.rights.urihttp://creativecommons.org/licenses/by/2.5/ca/*
dc.subjectneural precursor
dc.subjectpannexin
dc.subjectstroke
dc.subjectIsland Medical Program
dc.subject.departmentDivision of Medical Sciences
dc.subject.departmentSchool of Medical Sciences
dc.titlePannexin 1 Differentially Affects Neural Precursor Cell Maintenance in the Ventricular Zone and Peri-Infarct Cortexen_US
dc.typeArticleen_US

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