Fringe dielectrophoresis nanoaperture optical trapping with order of magnitude speed-up for unmodified proteins

Date

2023-03-31

Authors

Babaei, Elham
Wright, Demelza
Gordon, Reuven

Journal Title

Journal ISSN

Volume Title

Publisher

Nano Letters

Abstract

Single molecule analysis of proteins in an aqueous environment without modification (e.g., labels or tethers) elucidates their biophysics and interactions relevant to drug discovery. By combining fringe-field dielectrophoresis with nanoaperture optical tweezers we demonstrate an order of magnitude faster time-to-trap for proteins when the counter electrode is outside of the solution. When the counter electrode is inside the solution (the more common configuration found in the literature), electrophoresis speeds up the trapping of polystyrene nanospheres, but this was not effective for proteins in general. Since time-to-trap is critical for high-thoughput analysis, these findings are a major advancement to the nanoaperture optical trapping technique for protein analysis.

Description

Keywords

optical tweezers, dielectrophoresis, single protein analysis, nanoaperture tweezers, plasmonics, nanophotonics

Citation

Babaei, E., Wright, D., & Gordon, R. (2023). Fringe dielectrophoresis nanoaperture optical trapping with order of magnitude speed-up for unmodified proteins. Nano Letters, 23(7), 2877–2882. https://doi.org/10.1021/acs.nanolett.3c00208