Fringe dielectrophoresis nanoaperture optical trapping with order of magnitude speed-up for unmodified proteins

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dc.contributor.authorBabaei, Elham
dc.contributor.authorWright, Demelza
dc.contributor.authorGordon, Reuven
dc.date.accessioned2023-04-27T21:14:23Z
dc.date.copyright2023en_US
dc.date.issued2023-03-31
dc.description.abstractSingle molecule analysis of proteins in an aqueous environment without modification (e.g., labels or tethers) elucidates their biophysics and interactions relevant to drug discovery. By combining fringe-field dielectrophoresis with nanoaperture optical tweezers we demonstrate an order of magnitude faster time-to-trap for proteins when the counter electrode is outside of the solution. When the counter electrode is inside the solution (the more common configuration found in the literature), electrophoresis speeds up the trapping of polystyrene nanospheres, but this was not effective for proteins in general. Since time-to-trap is critical for high-thoughput analysis, these findings are a major advancement to the nanoaperture optical trapping technique for protein analysis.en_US
dc.description.embargo2024-03-31
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThe authors acknowledge funding from the NSERC (Canada) Discovery Grant RGPIN-2017-03830. The authors acknowledge the support of CMC for COMSOL simulations.en_US
dc.identifier.citationBabaei, E., Wright, D., & Gordon, R. (2023). Fringe dielectrophoresis nanoaperture optical trapping with order of magnitude speed-up for unmodified proteins. Nano Letters, 23(7), 2877–2882. https://doi.org/10.1021/acs.nanolett.3c00208en_US
dc.identifier.urihttps://doi.org/10.1021/acs.nanolett.3c00208
dc.identifier.urihttp://hdl.handle.net/1828/15014
dc.language.isoenen_US
dc.publisherNano Lettersen_US
dc.subjectoptical tweezersen_US
dc.subjectdielectrophoresisen_US
dc.subjectsingle protein analysisen_US
dc.subjectnanoaperture tweezersen_US
dc.subjectplasmonicsen_US
dc.subjectnanophotonicsen_US
dc.titleFringe dielectrophoresis nanoaperture optical trapping with order of magnitude speed-up for unmodified proteinsen_US
dc.typePostprinten_US

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