Time-resolved fluorescence anisotropy as a tool to study guest-cucurbit[n]urilprotein ternary supramolecular interactions
Date
2015
Authors
Scholtbach, Karina
Venegas, Italo
Bohne, Cornelia
Guentealba, Denis
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Journal ISSN
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Publisher
Photochemical & Photobiological Sciences
Abstract
Ternary supramolecular complexes involving cucurbit[n]urils and proteins are of potential interest for improving drug transport and delivery. We report here time-resolved fluorescence studies for acridine orange complexes with cucurbit[7]uril and cucurbit[8]uril in the presence of human serum albumin as a model system. A detailed characterization of the fluorescence lifetime and anisotropy properties of the different acridine orange complexes with cucurbit[n]urils and human serum albumin was performed. Of particular importance is the analysis of the stepwise binding for acridine orange–cucurbit[8]uril complexes and the assignment of the fluorescence and anisotropy properties to the 2 : 1 complex. Anisotropy decay measurements were essential to detect protein-bound species and to discriminate between different complexes. Based on the fluorescence evidence, ternary interactions with the protein are suggested for the acridine orange–cucurbit[7]uril complex but not for the cucurbit[8]uril complex. We highlight here the usability and sensitivity of the combined fluorescence analysis.
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Citation
Scholtbach, K., Venegas, I., Bohne, C., & Guentealba, D. (2015). Time-resolved fluorescence anisotropy as a tool to study guest-cucurbit[n]uril-protein ternary supramolecular interactions. Photochemical & Photobiological Sciences, 14, 842-852. https://doi.org/10.1039/C4PP00479E.