Time-resolved fluorescence anisotropy as a tool to study guest-cucurbit[n]urilprotein ternary supramolecular interactions

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dc.contributor.authorScholtbach, Karina
dc.contributor.authorVenegas, Italo
dc.contributor.authorBohne, Cornelia
dc.contributor.authorGuentealba, Denis
dc.date.accessioned2020-08-22T00:12:29Z
dc.date.available2020-08-22T00:12:29Z
dc.date.copyright2015en_US
dc.date.issued2015
dc.description.abstractTernary supramolecular complexes involving cucurbit[n]urils and proteins are of potential interest for improving drug transport and delivery. We report here time-resolved fluorescence studies for acridine orange complexes with cucurbit[7]uril and cucurbit[8]uril in the presence of human serum albumin as a model system. A detailed characterization of the fluorescence lifetime and anisotropy properties of the different acridine orange complexes with cucurbit[n]urils and human serum albumin was performed. Of particular importance is the analysis of the stepwise binding for acridine orange–cucurbit[8]uril complexes and the assignment of the fluorescence and anisotropy properties to the 2 : 1 complex. Anisotropy decay measurements were essential to detect protein-bound species and to discriminate between different complexes. Based on the fluorescence evidence, ternary interactions with the protein are suggested for the acridine orange–cucurbit[7]uril complex but not for the cucurbit[8]uril complex. We highlight here the usability and sensitivity of the combined fluorescence analysis.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThe authors thank CONICYT for the financial support through their FONDECYT research program (Grant no. 11121223) and the Pontificia Universidad Catolica de Chile through VRA for the partial financing of a research stay at the University of Victoria. The research at the University of Victoria was supported by a Discovery Grant from the Natural Sciences and Engineering Council of Canada (NSERC) to CB.en_US
dc.identifier.citationScholtbach, K., Venegas, I., Bohne, C., & Guentealba, D. (2015). Time-resolved fluorescence anisotropy as a tool to study guest-cucurbit[n]uril-protein ternary supramolecular interactions. Photochemical & Photobiological Sciences, 14, 842-852. https://doi.org/10.1039/C4PP00479E.en_US
dc.identifier.urihttps://doi.org/10.1039/C4PP00479E
dc.identifier.urihttp://hdl.handle.net/1828/12016
dc.language.isoenen_US
dc.publisherPhotochemical & Photobiological Sciencesen_US
dc.subject.departmentDepartment of Chemistry
dc.titleTime-resolved fluorescence anisotropy as a tool to study guest-cucurbit[n]urilprotein ternary supramolecular interactionsen_US
dc.typePostprinten_US

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