Mechanism of a disassembly-driven sensing system studied by stopped-flow kinetics
Date
2021
Authors
Gallo, Cara
Thomas, Suma S.
Selinger, Allison J.
Hof, Fraser
Bohne, Cornelia
Journal Title
Journal ISSN
Volume Title
Publisher
The Journal of Organic Chemistry
Abstract
We carried out steady-state and stopped-flow photophysical measurements to determine the kinetics of a discrete
disassembly driven turn-on fluorescent system. On and off rates for both DimerDye1 (DD1) assembly, and nicotine binding were
determined. Relative rates for these competing processes provide insight on how this system can be optimized for sensing applications.
Kinetics studies in artificial saliva showed that moving to more complex media has minimal effects on the sensing ability of
the system.
Description
Keywords
Anatomy, Fluorescence, Kinetic parameters, Kinetics, Nicotine, Centre for Advanced Materials and Related Technology (CAMTEC)
Citation
Gallo, C., Thomas, S. S., Selinger, A. J., Hof, F., & Bohne, C. (2021). Mechanism of a disassembly-driven sensing system studied by stopped-flow kinetics. The Journal of Organic Chemistry, 86(15), 10782-10787. https://doi.org/10.1021/acs.joc.1c00959