Labeling of proteins by BODIPY-quinone methides utilizing anti-Kasha photochemistry
Date
2020
Authors
Zlatić, Katarina
Antol, Ivana
Uzelac, Lidija
Dražić, Ana-Matea M.
Kralj, Marijeta
Bohne, Cornelia
Basarić, Nikola
Journal Title
Journal ISSN
Volume Title
Publisher
ACS Applied Materials & Interfaces
Abstract
A novel approach for the photolabeling of proteins
by a BODIPY fluorophore is reported that is based on an anti-Kasha
photochemical reaction from an upper singlet excited state (Sn)
leading to the deamination of the BODIPY quinone methide precursor.
On the other hand, the high photochemical stability of the
dye upon excitation by visible light to S1 allows for the selective
fluorescence detection from the dye or dye-protein adduct, without
concomitant bleaching or hydrolysis of the protein-dye adduct.
Therefore, photolabeling and fluorescence monitoring can be uncoupled
by using different excitation wavelengths. Combined theoretical
and experimental studies by preparative irradiations, fluorescence
and laser flash photolysis fully disclose the photophysical
properties of the dye and its anti-Kasha photochemical reactivity.
The application of the dye was demonstrated on photolabeling of
bovine serum albumin.
Description
Keywords
BODIPY, Fluorescence, Proteins, Photochemistry, Quinone methide, Centre for Advanced Materials and Related Technology (CAMTEC)
Citation
Zlatić, K., Antol, I., Uzelac, L., Dražić. A. M., Kralj, M., Bohne, C., & Basarić, N. (2020). Labeling of proteins by BODIPY-quinone methides utilizing anti-Kasha photochemistry. ACS Applied Materials & Interfaces, 12(1), 347-351. https://doi.org/10.1021/acsami.9b19472