Labeling of proteins by BODIPY-quinone methides utilizing anti-Kasha photochemistry

Simple item page
dc.contributor.authorZlatić, Katarina
dc.contributor.authorAntol, Ivana
dc.contributor.authorUzelac, Lidija
dc.contributor.authorDražić, Ana-Matea M.
dc.contributor.authorKralj, Marijeta
dc.contributor.authorBohne, Cornelia
dc.contributor.authorBasarić, Nikola
dc.date.accessioned2023-01-13T18:49:31Z
dc.date.available2023-01-13T18:49:31Z
dc.date.copyright2020en_US
dc.date.issued2020
dc.description.abstractA novel approach for the photolabeling of proteins by a BODIPY fluorophore is reported that is based on an anti-Kasha photochemical reaction from an upper singlet excited state (Sn) leading to the deamination of the BODIPY quinone methide precursor. On the other hand, the high photochemical stability of the dye upon excitation by visible light to S1 allows for the selective fluorescence detection from the dye or dye-protein adduct, without concomitant bleaching or hydrolysis of the protein-dye adduct. Therefore, photolabeling and fluorescence monitoring can be uncoupled by using different excitation wavelengths. Combined theoretical and experimental studies by preparative irradiations, fluorescence and laser flash photolysis fully disclose the photophysical properties of the dye and its anti-Kasha photochemical reactivity. The application of the dye was demonstrated on photolabeling of bovine serum albumin.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThese materials are based on work financed by the Croatian Science Foundation (HRZZ IP-2014-09-6312 for NB and IP-2013- 5660 for MK). The authors thank Dr. M. Cindrić for the MALDI TOF/TOF analyses of fluorescently labeled BSA. NB thanks Professor P. Wan at the University of Victoria, Canada, BC for financial support. CB thanks the Natural Sciences and Engineering Research Council of Canada (NSERC) for financial support (RGPIN- 121389-2012).en_US
dc.identifier.citationZlatić, K., Antol, I., Uzelac, L., Dražić. A. M., Kralj, M., Bohne, C., & Basarić, N. (2020). Labeling of proteins by BODIPY-quinone methides utilizing anti-Kasha photochemistry. ACS Applied Materials & Interfaces, 12(1), 347-351. https://doi.org/10.1021/acsami.9b19472en_US
dc.identifier.urihttps://doi.org/10.1021/acsami.9b19472
dc.identifier.urihttp://hdl.handle.net/1828/14661
dc.language.isoenen_US
dc.publisherACS Applied Materials & Interfacesen_US
dc.subjectBODIPY
dc.subjectFluorescence
dc.subjectProteins
dc.subjectPhotochemistry
dc.subjectQuinone methide
dc.subjectCentre for Advanced Materials and Related Technology (CAMTEC)
dc.subject.departmentDepartment of Chemistry
dc.titleLabeling of proteins by BODIPY-quinone methides utilizing anti-Kasha photochemistryen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Bohne_Cornelia_ACSApplMaterInterfaces_2020.pdf
Size:
1.42 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Faculty and Staff Publications
 
University of Victoria Libraries

We acknowledge and respect the Lək̓ʷəŋən (Songhees and Esquimalt) Peoples on whose territory the university stands, and the Lək̓ʷəŋən and W̱SÁNEĆ Peoples whose historical relationships with the land continue to this day.